DNA Sequencing

Molecule Tutorials - Herong's Tutorial Examples

∟DNA Sequencing

This chapter provides notes and tutorial examples on DNA sequencing. Topics include PCR (Polymerase Chain Reaction), Sanger Sequencing using the chain-termination method, NGS (Next-Generation Sequencing).

What Is DNA Sequencing

What Is PCR (Polymerase Chain Reaction)

What Is Sanger Sequencing Method

What Is NGS (Next-Generation Sequencing)

Takeaways:

DNA sequencing, also called gene sequencing, is the process of determining the nucleic acid sequence – the order of nucleotides in DNA.
PCR (Polymerase Chain Reaction) is a chemical synthesis process developed by Kary Mullis to rapidly amplify sequences of a DNA.
Sanger Sequencing is based on the chain-termination method developed by Frederick Sanger to determine the sequence of nucleotide bases in a given DNA.
NGS (Next-Generation Sequencing) testing aligns DNA fragments against a reference genome and counts matches at each genome positions.
NGS testing on cells requires breaking cells and chromosomes to generate DNA fragments.